Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of P…

페이지 정보

작성자 관리자
댓글 0건 조회 523회 작성일 22-03-16 14:46


Sensors 201515(8), 17977-17989

Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity

Butaek Lim, Ji-In Park, Kyung Jin Lee, Jin-Won Lee, Tae-Wuk Kim, Young-Pil Kim

We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that enabled the phosphorylated peptides to be strongly attached on the carboxyl groups of the QD surface via metal coordination, thus leading to a significant FRET efficiency. As a result, protein kinase activity in intermixed solution was efficiently detected by QD-FRET via Zn(II) coordination, especially when the peptide substrate was combined with affinity-based purification. We also found that mono- and di-phosphorylation in the peptide substrate could be discriminated by the Zn(II)-mediated QD-FRET. Our approach is expected to find applications for studying physiological function and signal transduction with respect to protein kinase activity 


등록된 댓글이 없습니다.

Total 18건 1 페이지
  • RSS